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Adelaide hundred one of my top 5 knocks, says Cheteshwar Pujara

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first_imgCheteshwar Pujara rated the classy 123 at the Adelaide Oval as one of his best innings in Test cricket. Pujara scored his first hundred in Australia and 16th in Tests as India finished with 250 for 9 despite a top-order failure.After opting to bat, India lost KL Rahul, Murali Vijay, Virat Kohli and Ajinkya Rahane to poor shot selection but Pujara stood firm and shared crucial partnerships with Rohit Sharma (37), Rishabh Pant (25) and R Ashwin (25) to save India the blushes on the opening day of the first Test.Pujara waged a lone battle throughout the first day. He walked in at the fall of KL Rahul’s wicket and saw his colleagues falling to irresponsbile shots. But he was in no mood to give up and held firm to ensure India 250 on the board by stumps.Pujara was solid in defence and did not play those booming drives which cost the others their wickets. He was patient and stayed focused against a top quality bowling attack. Once he had settled down, Pujara played a few spanking shots – in fact, he even hit two sixes and had reached 123 before being brilliantly run out by Pat Cummins.Pujara’s was a knock of substance, class, grace and style. It was also a lot about acceleration and identifying the key moments to attack.A lot of his teammates would do well to learn from Pujara – most of them agreed the hundred at the Adelaide Oval was one of his finest. Pujara himself did not want to rate it but reckoned it had to be with the best he has played.advertisement”It has to be among the top five… I can’t rate it as one of the best but the way my teammates appreciated the knock.. they said it was one of the best,” Pujara told the media at the end of day’s play.There were several challenges Pujara had to deal with. First, the flurry of wickets at the other end and then the heat in Adelaide did not make run making any easier. And just when he settled into a nice partnership with Ashwin, Pat Cummins broke the resistance to peg India back further.”It was tough but I was set and I knew I could play my shots especially when we lost seven wickets. Me and Ash (Ashwin) had a good partnership but once we lost Ash, I thought I had to accelerate.. I knew what shots I could play on that wicket because I had batted for two sessions,” he said. “But yes it was tough considering the weather.. It was quiet hot.. We are used to it in India but still it was really hot.”Pujara was eventually run out for 123 on what turned out to the last ball of the day. India are now nine down and have their Nos. 10 and 11 to bat when play resumes on the second day. Pujara was disappointed he got out moments before stumps but said he had to go for the single.”I was a bit disappointed to get out but I had to take a single – only two balls were left in the day’s play.. so I thought I should be on strike in the morning,” he said.But Pujara has clearly done his job and it is now up to the bowlers to try and keep India in the game. And then perhaps the batsmen can take a leaf out of Pujara’s book and script a comeback.Also Read | Sunil Gavaskar blasts India batsmen after poor shot selection in Adelaide TestGallery | 1st Test, Day 1: Cheteshwar Pujara India’s lone bright spark as Australia bowlers dominateAlso watch –last_img read more

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FLOW Manchester United Ultimate Football Experience – Sat Apr 22nd

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first_img Recommended for you Facebook Twitter Google+LinkedInPinterestWhatsAppTurks and Caicos, April 25, 2017 – Providenciales – Join us for the Flow Ultimate Football Experience happening in Providenciales THIS weekend!Date: Saturday, 23rd April 2017Time: 8:30am – 12:30pmVenue:   TCI Football Association National Academy FieldDetails:   Flow and Manchester United Football Club have partnered to bring a great opportunity to footballers across 15 Caribbean countries. 32 of TCI’s top young footballers, aged 13 to 16, from the local football clubs/schools have been chosen to compete in three skills: control, short passing and dribbling.The skills are all taken from the Manchester United Soccer Schools’ programme and local coaches will evaluate and rank each player throughout the morning.The Results:   Based on the rankings and assessments in each skill, two winners will be selected at the end of the event. These two players along with their coaches and a parent will then travel to Trinidad & Tobago where the players will compete in a final weekend of skills and small sided games. The finals weekend will include one-on-one training with Caribbean Football Union (CFU) and Manchester United Soccer School Coaches.The players will also compete with the finalists from the other 14 countries for the chance to be one of two players to win a once-in-a-lifetime VIP trip to Old Trafford in Manchester, England. The coaches of the winning players will also attend.Contact: Darron D. Hilaire Jr. Marketing Communications Executive E: [email protected] T: 1-649-941-4478 ● M: 1-649-231-6275#Flowsports Related Items:#flowsports 2017 CARIFTA TEAM TCI Flow CARIFTA Games 2017: Exciting on-the-go access, more broadcast hours for Caribbean sports fans Facebook Twitter Google+LinkedInPinterestWhatsApp Flow CARIFTA Games 2017: Exciting on-the-go access, more broadcast hours for Caribbean sports fanslast_img read more

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Stagnito Buys Nielsens Food Magazines

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Apples minor update for iPhones iPads makes a huge difference

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Narayanganj road crash kills 8

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Biman resumes DhakaDelhi flights after 5 yrs

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Green Line pays Russel second instalment of Tk 500000

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CityWide Water Testing Planned

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Police Chief Apologizes for Officers Black Bad Guy Remark

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Nanocontainers for nanocargo Delivering genes and proteins for cellular imaging genetic medicine

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first_imgIn their paper, the authors report that the T4 capsid NP gene expression and protein delivery system may be complementary to or used in conjunction with gene therapy based on RNA Cas and taran nuclease. (Cas genes code for proteins related to DNA loci containing short repetitions of base sequences known as Clustered Regularly Interspaced Short Palindromic Repeats, or CRISPRs.) “The T4 nanoparticle expression system can easily complement Cas9 and taran nuclease-based recombination by packaging the linear cas9, target-sgRNA plasmid DNA, and Cre recombinase – or even ligase, an enzyme that facilitates the joining of DNA strands – and deliver the resulting T4 nanoparticles into the recipient eukaryotic cells with high specificity employing SOC and HOC,” Liu tells Phys.org. (SOC and HOC are dispensable T4 capsid proteins.) “By displaying the targeting ligands (binding molecules) onto the surface, the T4 capsid gene expression and protein system will be able to efficiently deliver the Cas9 and sgRNA plasmids together into the desired recipient cells. Relevant enzymatically-active proteins Cas9, lambda exonuclease, lambda beta protein and others can be delivered directly at the same time from the T4 nanoparticle.” Seamless gene correction of beta-thalassemia mutations in patient-specific cells Dr. Jinny L. Liu discussed the paper that she, Prof. Lindsay W. Black and their co-authors published in Proceedings of the National Academy of Sciences USA. “Icosahedral viral nanoparticles are essentially 100 nm by 80 nm nanocontainers that allow exogenous genetic material to be packaged in vitro through nucleic acid machinery that generally only allows linear DNA/RNA to be packaged through a portal channel,” Liu tells Phys.org. “However, in vitro protein packaging is generally impossible, because for most viral nanoparticles there is no protein packaging machinery comparable to nucleic acid packaging machinery.” While protein may be chemically cross-linked to the capsid inner surface, this is expected to lead to protein denaturation and loss of enzymatic activity.That being said, nature has evolved solutions to this protein packaging conundrum. During in vivo viral capsid assembly, Liu explains, some bacterial viruses, or bacteriophages, target proteins within the procapsids before the nucleic acid is packaged so as to eject the proteins with the nucleic acid, thereby facilitating infection in conjunction with the nucleic acid. (A procapsid, or prohead, is an immature viral capsid structure formed in the early stages of self-assembly of some bacteriophages. Production and assembly of stable proheads is an essential precursor to bacteriophage genome packaging.) Only a few phages have well-characterized in vivo protein packaging systems, and phage T4 is the best characterized. “Prof. Black’s lab at UMB and my lab at NRL have demonstrated that not only can a specific foreign enzyme – cyclic recombination (Cre) recombinase – be packaged into the capsid in vivo, but also that it is active within the capsid.” This activity was demonstrated by showing the religation (the rejoining of two DNA strands or other molecules by a phosphate ester linkage) of packaged linear DNA flanked with two Cre recombination sites.The paper shows that the substantial space within a T4 nanocontainer accommodates the active Cre enzyme along with exogenous DNA. “For potential applications, T4 can package up to 50 kb exogenous linear DNA containing full-length desired genes along with recombinases, either Cre or λ-red proteins, for specific homologous recombination within the chromosome,” Liu notes. (Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA.) “We expect that the cas9 enzyme could be encapsidated in a comparable way – and in fact, at least eight different proteins have been encapsidated in this manner. Through homologous recombination, our system can allow the corrected gene to replace the mutated gene in its original location within the chromosome or by precisely knocking out the overactive genes in stem cells.” Liu points out that the T4 delivery vector is safer and better controlled than other viral delivery gene therapy, such as those delivering genes using infectious animal viral vectors to randomly insert the gene within the chromosome. Liu adds that her lab has also been studying cell imaging and drug/gene delivery to eukaryotic cells using T4 tailless nanoparticles, which the researchers demonstrated can enter the eukaryotic cells without causing cell death.A specific example of potential downstream drug and gene therapeutic applications resulting from the new approach is delivery of the toxic protein and linear plasmid that produces neutralizing peptides or antibodies into targeted cancer cells displaying specific cancer markers using high affinity SOC + HOC marker binding proteins on the surface of the capsids, while another example is to use the system for HIV gene therapy. Liu adds that there are several pathways to use this system for gene therapy:Delivering T4 nanoparticles packaged with the recombinase (or ligase) and linear plasmid DNA to produce gp120 or interferon to generate or boost the immune response in patientsDelivering T4 nanoparticles packaged with recombinase (ligase) and the linear soluble CD40 expression plasmid DNA into T lymphocytes or hematopoietic cells to block the infection of HIV-1Inhibiting RNA by delivering the engineered plasmid DNA that can produce decoy RNA for binding the viral sense DNAInhibiting protein by delivering packaged anti-viral antibodies and anti-HIV antibody plasmid DNA In addition to diagnostic and cellular imaging, the T4 nanoparticle gene-protein system can deliver repaired genes to correct human genetic diseases – for example, reversing adenosine deaminase (ADA) deficiency by introducing the protein-DNA complex to express ADA in stem cells. Other broad areas of research impacted by gene therapy technologies, such as genetic defects, cancer, neurological diseases in adults, and aging itself, may also benefit from this study.Moving forward, the scientists want to develop more T4 procapsids packaging exonuclease and other recombinases along with engineered target DNA to demonstrate that the resulting T4 capsids can insert the gene into a stem cell line with a genetic deficiency. “In addition,” Liu concludes, “we’re working on adapting our system to deliver therapeutic peptides or antibodies to cells exposed to or infected by biothreat agents, such as protein toxins or viruses, efficient neutralization of toxin effects. The treatment and cure of cells and tissues exposed to such agents are of a great interest to our biodefense research community.” Journal information: Proceedings of the National Academy of Sciences Explore further The T4 capsid-derived specific exogenous DNA plus protein packaging and eukaryotic cell delivery scheme. (A) DNA encoding a 10-amino acid N-terminal CTS peptide fused to the phage P1 Cre allows synthesis of CTS-Cre and targeting of the enzyme into the early core-scaffold of the T4 procapsid in vivo. Procapsid assembly and maturation-specific viral protease stabilize the procapsid, remove most of the scaffolding core as peptides, and remove the CTS peptide from Cre. Mutations in the viral terminase block DNA packaging and allow a mature but DNA-empty large Cre-containing procapsid to be highly purified from viral-infected bacteria. (B) In vitro packaging into the mature capsid of plasmid DNA containing mCherry driven by a CMV promoter and two loxP sites flanking an SfiI restriction enzyme site that allow the linearization required for packaging. The DNA is packaged into the procapsid by the ATP-driven terminase motor protein (gp17) with high efficiency. (C) The packaged Cre enzyme recircularizes the packaged linear plasmid DNA between the two loxP sites. The DNA-containing capsid is taken up by eukaryotic cells, here without displaying a specific peptide target, or into eukaryotic cells specifically using Soc and Hoc displayed peptides that have high affinity for the RP1 and RP2 receptors, respectively. Credit: Liu JL, et al. (Published online before print August 26, 2014) Viral nanoparticle-encapsidated enzyme and restructured DNA for cell delivery and gene expression. Credit: Liu JL, et al. (2014) Viral nanoparticle-encapsidated enzyme and restructured DNA for cell delivery and gene expression. Proc Natl Acad Sci USA Published online before print August 26, 2014. doi:10.1073/pnas.1321940111 More information: Viral nanoparticle-encapsidated enzyme and restructured DNA for cell delivery and gene expression, Proceedings of the National Academy of Sciences USA, Published online before print August 26, 2014, doi:10.1073/pnas.1321940111 Measurement of the inhibition by endocytosis inhibitors and colocalization with lysosomes in A546-T4–treated A549 cells. (A) Pretreatment with amantadine, specifically stabilizing the clathrin-coated pits, reduced the uptake of A546-T4 NPs by A549 cells in a concentration-dependent manner. (B) Pretreatment with the PI3 kinase inhibitor, wortmannin, also reduced the uptake of A546-T4 in a concentration-dependent manner. (C) An overlapping confocal cell image obtained with a 60× objective with the internalized A546-T4 procapsids (yellow), lysosomes stained with LysoTracker Blue (blue), and the overlapping spots (white). (Scale bar, 10 μm.) (D) A confocal image shows the broad view of treated cells containing overlapping portions (white spots) of lysosomes (blue) with A546-T4 procapsids (yellow). The image was obtained using a 20× objective. (Scale bar, 50 μm.) Credit: Liu JL, et al. (Published online before print August 26, 2014) Viral nanoparticle-encapsidated enzyme and restructured DNA for cell delivery and gene expression. Credit: Liu JL, et al. (2014) Viral nanoparticle-encapsidated enzyme and restructured DNA for cell delivery and gene expression. Proc Natl Acad Sci USA Published online before print August 26, 2014. doi:10.1073/pnas.1321940111 (Phys.org) —By loading any specific protein and nucleic acid into an icosahedral phage T4 capsid-based nanoparticle, the resulting cell delivery vehicle’s ligands can bind to the surface of specific target tissues to deliver the protein/DNA cargo. (Icosahedral viral nanoparticles are evolutionary protein shells assembled in a hierarchical order that results in a stable protein layer and an inner space for accommodating nucleic acids and proteins; a capsid is the protein shell of a virus.) The technique has drug- and gene-delivery applications in human diseases, diagnostic and cellular imaging, and other medical areas. Recently, scientists at US Naval Research Laboratory, Washington, DC and University of Maryland at Baltimore packaged T4 nanoparticles in vivo with active cyclic recombination, or Cre, recombinase (a genetic recombination enzyme used to manipulate genome structure and control gene expression) and in vitro with fluorescent mCherry (a fluorescent protein used as a marker when tagged to molecules and cell components) expression plasmid DNA, and delivered these nanoparticles into cancer cells: When released into cells in the presence of both DNA and protein, the recombinase enhances mCherry expression by circularization (that is, changing the packaged linear DNA into a circular loop). The researchers state that this efficient and specific packaging into capsids and the unpackaging of both DNA and protein with release of the enzymatically altered protein/DNA complexes from the nanoparticles into cells have potential in numerous downstream applications such as genetic and cancer therapeutics. © 2014 Phys.org Citation: Nanocontainers for nanocargo: Delivering genes and proteins for cellular imaging, genetic medicine and cancer therapy (2014, September 16) retrieved 18 August 2019 from https://phys.org/news/2014-09-nanocontainers-nanocargo-genes-proteins-cellular.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more

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